Surely it’s recommended that Water could be sterilized or free from all the Contamination for many protocols and experiment as the contaminated water can cause the severe changes in the experiments or it can change the products of the experiments or can produces the error in the experiments, The goal of each chemical/clinical/research laboratories is to produced accurate results, that can be a major problem for the lab workers.
Inadequate control of contamination in water is an important potential cause of laboratory and similar forms of radiation are either costly or underdeveloped or are not readily available. Autoclaving Process is the safest, most economical, and efficient method of obtaining sterile water. Ultraviolet light is relatively ineffective against spores. Water in small amounts of 1 to 2 liters can be easily and safely heat sterilized in either an open or closed vessel this method of obtaining sterile solutions is used throughout the world. Filtration does not remove all the contaminations. Autoclaves work by allowing steam to enter and maintaining extremely high pressure for at least 15 minutes.
This means that all bacteria, viruses, fungi, and spores are destroyed. Autoclave uses steam for the sterilization equipment by subjecting the articles to high pressure saturated steam at 121 °C and 15 pressure pounds per square inch depending, for around 15 20″ minutes, on the size of the load and the contents. Cap must be slightly loose that pressure created throughout the cycle does not cause the vessel to break, if the container containing cap. Liquids to be autoclaved must be in an autoclavable vessel that is at least twice as large as the volume to be autoclaved If you are autoclaving 1 liquid liter, it could be put in a flask that hold at least 2 liters. For autoclaving water, it shouldn’t be placed in a sealed container in the autoclave it might explode and can cause damage.
There going to be always few inches of head room in the containers of the water or liquid articles may be free.